Journal: Shock (Augusta, Ga.)
Article Title: Cardiac Progenitors Induced from Human Induced Pluripotent Stem Cells with Cardiogenic Small Molecule Effectively Regenerate Infarcted Hearts and Attenuate Fibrosis
doi: 10.1097/SHK.0000000000001133
Figure Lengend Snippet: According to treatment outline, the CPCs expressed CM, EC, SMC specific proteins. (A) CM progenitors expressed α-sarcomeric actinin, cTnI, MLC2v, cTnT and Cx43, scale bar = 50 μm. (B) Under TEM, these cells were rich in endoplasmic reticulum (ER) studded with ribosomes, developing myofilaments (mf), mitochondria (arrowhead) and glycogen particles. Nuclear chromatin material (N) was uniformly distributed in the nucleoplasm. (C) Endothelial progenitors expressed VE-cadherin and CD31, scale bar = 200 μm. LDL uptake by these cells was observed (D) and these also formed tube like structures (E). (F) Smooth muscle progenitors expressed a-SMA and calponin, scale bar = 200 μm. (G) FACS analysis revealed 95.2 ± 2.1% CMS, 90.3 ± 2.5% ECs and 92.3 ± 1.8% SMCs in basal differentiation medium.
Article Snippet: For endothelial cells (ECs) differentiation, culture medium was switched to EGM-2V medium (Lonza) for another 10days.
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